FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4481315
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA16950.055.2198562
CGAGTAG16650.051.890593
GAGTAGC42450.046.4723364
AGTAGCT66750.045.5189825
CCCGAGT22800.043.8951071
TTGGGAG103350.043.7502446
TACTCGG19850.043.162653
GCTGGGA139250.042.1916289
GTAGCTG71350.041.6761326
CTACTCG20150.041.4480932
TAGCTGG79850.039.8546187
GGGAGGC137650.039.0729068
GGAGGCT110950.039.0012449
TCGCGTT2057.2759576E-1238.634045
TCGGGAG24450.037.9876256
CTCGGGA23450.036.2301755
TGGGAGG141500.034.6006167
GCTACTC50600.033.7236061
TTTGGGA111750.032.5368235
GGAGGTT60650.032.0526778
Produced by FastQC (version 0.11.5)