FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4758971
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1457.2759576E-1249.653035
CCGAGTA17550.044.3220752
CGAGTAG16400.043.9005433
CGGAGGT16850.038.4553157
GCTGGGA126850.037.232929
AGTAGCT65350.037.0175325
CCCGAGT25250.035.7136961
GAGTAGC43000.035.6635784
TTGGGAG93300.034.5708546
GTAGCTG70750.033.8868756
TAGCTGG75700.033.5731857
TACTCGG18000.033.1985663
CTACTCG17450.033.019312
CGCGTTG2206.730261E-1032.725866
GGAGGCT102700.032.3880849
GGGAGGC118950.032.2003758
GCTACTC45150.030.678181
GGAGGTT51100.030.0104478
CGGGTTC14200.029.4071839
CTCGCGT3201.8189894E-1229.2487354
Produced by FastQC (version 0.11.5)