FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4758971
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA16650.041.9464072
CGAGTAG16950.039.9292263
TACTCGG18050.039.4903263
AGTAGCT63000.039.0857355
CTACTCG17350.038.5940862
GCTGGGA128450.038.3414429
GAGTAGC41650.037.166894
TTGGGAG100750.036.803996
CGGAGGT21300.035.4929777
CCCGAGT24450.034.748851
GGAGGCT107800.034.5317239
GTAGCTG71850.033.7703746
GGGAGGC126550.033.453998
TAGCTGG74700.033.44587
GCTACTC47150.032.984461
GGAGGTT55300.032.289358
TCGGGAG23850.031.6981326
CTCGGGA23750.030.6189655
TGGGAGG134350.030.1183647
AGGCGGA30350.029.6540554
Produced by FastQC (version 0.11.5)