FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4554581
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA15150.043.7354972
CGAGTAG14400.042.998823
AGTAGCT63850.038.451575
GCTGGGA118800.037.8777479
GAGTAGC37350.036.8182644
TTGGGAG93850.036.5168466
CGGAGGT19750.036.4546937
GTAGCTG67900.035.733926
GGAGGTT49250.035.0853358
TAGCTGG72800.034.812237
TACTCGG15450.033.552483
GGAGGCT99250.033.4419069
CTACTCG16400.033.3755652
CCCGAGT24050.033.2908941
GGGAGGC117050.032.4160278
TGGGAGG123400.030.8062887
TCGGGAG20400.029.2933146
GCTACTC41150.028.571651
CTCGGGA19500.028.4299895
GGCGGAG30500.028.0910325
Produced by FastQC (version 0.11.5)