FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4554581
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA14700.043.5909542
GAGTAGC39450.042.8888664
CGAGTAG14400.042.4991383
AGTAGCT65350.039.111685
CTACTCG15650.038.1845322
TTGGGAG95500.037.770966
TACTCGG16550.037.4131433
GCTGGGA123900.037.1905339
GGAGGCT103350.036.156029
GTAGCTG68450.035.8677946
CCCGAGT21400.035.3275531
GGGAGGC121750.035.008748
GCTACTC40750.034.631121
TAGCTGG72200.034.5034567
CGGAGGT22350.034.146967
TCGGGAG20300.032.6298836
CTCGGGA18900.031.9993555
TGGGAGG128650.031.7319687
GGAGGTT54250.031.3210268
AGGCGGA27900.030.1929384
Produced by FastQC (version 0.11.5)