FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4925301
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19550.050.4535873
CCGAGTA21700.050.448942
AGTAGCT75550.042.9793975
GAGTAGC46700.040.855194
CCCGAGT29550.039.2941551
GTAGCTG80800.038.9393166
GCTGGGA151650.038.740579
TAGCTGG84200.038.2220237
TTGGGAG107950.037.616186
GGAGGCT121250.034.9151469
GCTACTC50300.033.4077721
TACTCGG20200.033.1474273
CTACTCG19650.032.9874082
CGGAGGT25650.032.8410267
GGGAGGC147900.031.8853668
TGGGAGG149050.030.8664827
TCGGGAG25900.030.0221826
GGAGGTT62900.029.6460868
GCACTTT102100.029.102551
TTTGGGA116450.028.7495965
Produced by FastQC (version 0.11.5)