FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4229857
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22300.065.217973
CCGAGTA23400.064.935152
AGTAGCT87750.060.224025
GAGTAGC55950.059.451484
GTAGCTG91600.056.5137636
TAGCTGG104100.052.1484537
GCTGGGA181200.051.7337079
CCCGAGT32300.049.7923931
TTGGGAG121550.048.7489976
CTACTCG22850.046.3280832
TACTCGG23950.042.9884033
CCAAGTA44550.042.0280152
CAAGTAG42850.041.837853
GGAGGCT138300.040.762469
GCACTTT104700.040.7100371
AAGTGCT107650.039.9283265
TTTGGGA122200.039.8876345
AAGTAGC47200.039.507414
GCTACTC57700.038.997741
GGGAGGC179850.038.6311538
Produced by FastQC (version 0.11.5)