FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4229857
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT78500.063.2868845
CGAGTAG19700.063.227943
GAGTAGC50300.059.832544
CCGAGTA21500.058.9390832
GTAGCTG87350.056.0506066
TTGGGAG133850.052.5545656
GCTGGGA182300.051.3046539
CCCGAGT28000.050.1436541
TAGCTGG101000.048.618027
GCACTTT110800.046.59281
CTACTCG27050.046.3138772
GGAGGCT149250.046.2152639
TCGGGAG30400.045.9475566
TTTGGGA127700.045.8952565
TACTCGG27500.045.817833
GGGAGGC197400.043.6232768
GCTACTC62100.043.4789541
CAAGTAG40050.043.1457373
CACTTTG118250.042.8647842
AAGTGCT107850.042.5259065
Produced by FastQC (version 0.11.5)