FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3195774
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG1851.749598E-594.92127270-274
GATTCGT2500.00649193153.41027275-279
CTCGCGT1451.5643309E-1052.13903210-214
AAACGAA8000.00800781950.629997295
CGAGTAG12750.049.8757363
GCGTTAT2500.00945208448.56893240-244
TACGTCC1906.6429675E-547.87548285-289
CGAAATC4550.047.506973105-109
CTACTCG10750.045.1898082
AGTAGCT53250.045.0124475
CCGAGTA14200.044.973482
GCTGGGA103350.043.754889
GTAGCTG54350.043.3014036
TTGGGAG76850.043.0004846
TATAGCG3151.1403878E-542.861374295
GAGTAGC35300.042.803944
TACTCGG11300.042.3270383
TAGCTGG60850.041.4448937
ACCGATC2051.190572E-441.110676255-259
CCCGAGT17700.039.7897261
Produced by FastQC (version 0.11.5)