FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4125804
Sequences flagged as poor quality0
Sequence length41-301
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG750.00418796861396.3965275-279
CGTACGG400.0047712071309.1218275-279
CGACCGG1350.00539411931229.4076295
CGACCGA1000.008346562989.1143155-159
GTCGGTA1050.008652045971.4515235-239
CCGTCGA1100.009495359927.2946235-239
CGGTATG2554.3252287E-5793.8172225-229
AGCATCG6351.0362637E-6478.1655220-224
CGGTAGG2601.2248276E-4382.35965190-194
TTAGCGG2900.0045889085355.4349260-264
TGTCGAT3457.6778163E-4304.37833280-284
CGGGCAA3751.4901865E-5267.13675200-204
TCGCGCC3901.6292724E-6260.8653230-234
CGCGCCT3801.0913936E-11257.6903105-109
CGGGTAG3953.3800976E-4252.96387195-199
AGTGCGG4054.2584186E-4249.90541225-229
GCGGGTT4150.006151714247.72429250-254
CTGCGTT7000.0076659066237.10002295
TCCTCGG8551.5854372E-5236.7525220-224
ACCTCGC4300.0016326207236.59875230-234
Produced by FastQC (version 0.11.5)