FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4125804
Sequences flagged as poor quality0
Sequence length41-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTT950.00269146241740.5891295
TACGCGT750.0043557321369.2635270-274
TTCGCGC850.0053322061237.4825290-294
CGCACGA850.00595361651171.1888210-214
TCGCGCG450.0059858561168.7334290-294
ATTCGCG850.0061683581150.6416120-124
CGTCGAG900.00640451351129.1669235-239
CGCGCGA950.00709850671072.5302240-244
CCGGTCG1000.0076203891035.1196280-284
CGCGTTC1000.00774227361026.9476270-274
GACGCGA1000.008281608992.9774195-199
CCGGTTA2555.64684E-6648.4548295
GGTCGAA3301.0462546E-4591.2728115-119
CTCGCGT1954.370322E-7525.25757250-254
CGGGCCG4001.5587085E-4517.5598280-284
AATCGCC4101.599438E-4513.1025290-294
TCGCGTT1001.3348217E-6512.12616250-254
CGTTCGC1152.3226629E-4453.9655285-289
CGACAGG4502.552256E-4439.0887170-174
CGCAGTT5353.5505305E-4393.21872290-294
Produced by FastQC (version 0.11.5)