FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3482633
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG3156.5164483E-4321.4578295
ACGCTAG1907.4325874E-4307.84055140-144
GTCGGAT2700.001635818236.52718265-269
GTTGCGA1450.0010340017205.43022190-194
CGCGGTT1100.0028245805197.19473275-279
TTAGTCG1200.0036645427180.76184275-279
TCGCGGG1850.002747196177.1298285-289
CCTGCGA3700.0041170255173.75269270-274
GTCGGTT1906.9069756E-6171.6345280-284
CCCGAAA3600.0045167534168.4618225-229
CGCTAGA1850.005470739158.08029140-144
TGACGAT3950.0057394006155.49245240-244
GTCGCTC3850.005994795153.25046170-174
CGAGGTA4100.0060725985152.583250-254
CCCGTTA2002.3722883E-4145.97105125-129
TACGAAC2806.6702523E-7115.9058275-279
TACGGCC3000.005067109108.70184280-284
CCCAACG4700.0013835318107.72256295
GCAATCG3100.0064673973106.32647290-294
GTATTCG2800.0012090921104.3554135-139
Produced by FastQC (version 0.11.5)