FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4026650
Sequences flagged as poor quality0
Sequence length45-301
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG300.0045165251345.7358285-289
CGACGTA350.006070611160.7196290-294
GTCGCGA853.2602788E-5873.7189170-174
TCGCGAT1004.24329E-4371.3305170-174
GGCGTAC2154.4356444E-4365.68112265-269
CCGTAAC2457.280749E-4309.93546230-234
CTCGCGT1601.6166323E-6253.90741290-294
AGCGAAC2900.0013266972253.6672120-124
CCGAACG1600.0010396821237.75873235-239
CCGCGTG3550.0019435758223.27492270-274
TCGAGGG5502.2545957E-5221.59193290-294
ACTCGCG1700.0016047705218.42972170-174
CCGCGCT3500.0023031358210.9798135-139
CGCGTGC2953.276699E-5201.92676270-274
TGCCGTA4000.0026857015200.40363275-279
CCGGCGA2050.0037452613179.42314110-114
TGACCGG4700.004823944164.78047255-259
TCGAAAC4800.0053078104159.59895245-249
ACTCGTG5100.0055038994157.66695280-284
AATCCGA4800.005741763155.46347185-189
Produced by FastQC (version 0.11.5)