FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4026650
Sequences flagged as poor quality0
Sequence length45-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC1451.681425E-4505.49966115-119
ACGTCGC1004.3823617E-4367.35614130-134
CTCGCGT1702.8025752E-6218.68411195-199
TGCGCGT1250.002300893211.17659275-279
CGTGCCG2150.0034495543184.41696280-284
CGTGTAA4100.0035192308183.10504220-224
GGCGGTC4400.0037008554180.0414280-284
GCGGTCG2400.0047937064165.20686280-284
GTCGGTT2409.907656E-4165.03793280-284
ACCTGCG4950.0052639167160.03679280-284
CGTCGCA950.005768696155.33917145-149
GTGCGCG2550.0057730554155.25044275-279
GTCGCAT2450.006325342150.58388145-149
ACGTAAT5100.0067992094146.91737215-219
GCGCGTT1400.0076356595141.42494275-279
CGCGGGT3003.6335847E-4129.25494260-264
ACGGTCA6200.009975839129.22475290-294
CGTGCGC3250.0021881335121.749695275-279
ACGGGTT5300.00432168115.64874295
GACCCGC3400.0025742468112.58343250-254
Produced by FastQC (version 0.11.5)