FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3190629
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC2209.2859165E-487.22085285-289
CCGGTTA2250.002458520268.249794165-169
CGAGTAG15350.058.65533
CCGAGTA16650.057.089382
AGTAGCT61150.053.515265
GTAGCTG61350.052.8704226
GAGTAGC39100.051.3681034
TAGCTGG65500.051.106697
GCTGGGA113250.049.5867549
CAACGAA7754.7654114E-448.677765295
CTACTCG12400.048.611312
CCCGAGT20700.046.7678071
TTGGGAG82500.044.5632176
AAAGTGC61200.043.852324
AAGTGCT64100.043.489075
TACTCGG13700.043.3918043
GGAGGCT81800.041.769529
CCCAAAG75800.041.603571
AGCGTCG2350.00422687241.58143130-134
GCTACTC29700.041.1838461
Produced by FastQC (version 0.11.5)