FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319187
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGT2900.00331972663.26751265-269
GCACGAA4550.003597139661.990864295
CGAGTAG15800.061.8850563
CGTGCCG2550.004019840660.28612150-154
GCTCGAA4850.004632046458.156384295
CCGAGTA17350.057.590392
CTCGCGT1106.134287E-753.889587250-254
CTACTCG13000.052.8697172
GAGTAGC41450.052.035614
AGTAGCT64500.051.720445
ACGAATG5500.00761836151.28335295
TACTCGG14400.049.5282673
GCTACTC34000.048.7624131
GTAGCTG67700.048.681056
TAGCTGG70450.048.4952747
GCTGGGA129700.048.3814359
CCCGAGT21800.048.2277641
TTGGGAG93700.046.7745176
AAAGTGC67600.045.1795354
CCAAAGT77800.044.0228842
Produced by FastQC (version 0.11.5)