FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319187
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG16050.068.734642
TACTCGG16900.065.617563
GCTACTC37700.058.688631
CGAGTAG17100.057.7938733
AGTAGCT64550.056.3528445
GAGTAGC41600.054.6987654
TTGGGAG105300.054.4177556
TCGGTAT3500.006244458753.934727290-294
GTAGCTG67150.053.4087526
CCGAGTA18800.052.8734552
GCTGGGA140300.052.5090189
CCCGAGT20300.051.2409441
TAGCTGG73150.051.085467
GCACTTT82850.050.2898181
AAGTGCT77300.050.2545745
AAAGTGC75900.050.0420764
GGAGGCT108850.049.8225179
ACTTTGG84950.049.037023
TCGGGAG24500.049.0291186
ACTCGGG23350.048.4791534
Produced by FastQC (version 0.11.5)