FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3656432
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGC1953.9128473E-4108.40214285-289
GGTCGAT2157.4841885E-492.08878255-259
CGCTAGC1951.08992026E-769.372635265-269
ACGCTAG2251.6894717E-460.072052265-269
TTGCGAG4701.7026634E-459.967144285-289
GCACGAA5400.0052346956.386837295
CGAGTAG16600.054.3665963
GAGTAGC42300.052.2716184
AGTAGCT70750.051.5022665
CTACTCG12550.050.9782872
CCGAGTA18250.050.257712
GCTGGGA140300.050.013019
TAGCTGG74300.049.648857
GTAGCTG72550.049.524756
GCTACTC32550.048.54061
CCCAAAG93800.047.7557331
TACTCGG13250.047.6784823
AAAGTGC75150.047.1358034
CCAAAGT83300.046.5581932
TTGGGAG101050.046.4416126
Produced by FastQC (version 0.11.5)