FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4118179
Sequences flagged as poor quality0
Sequence length42-301
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG550.00137404312437.184270-274
CGCGAAC400.00309472791625.297195-199
CCGCGTA450.00407488971416.38195-99
AGTCGCG1100.0058793721178.1073185-189
CGATACG1200.00695010651083.5314205-209
CGGTCGC1250.00779496651023.1378135-139
CGTCGTA650.0081699281000.18286225-229
CCGCGAA1300.0081563061000.18286195-199
CTAAGCG2150.008446864621.3305265-269
GGAGCGT5602.2625842E-4456.75797120-124
TTACGGA3306.4731925E-5387.55222125-129
CCCAACG5102.9393763E-5386.2857295
GGGTCGC3851.3299323E-6354.256280-284
ATGTCGC3950.009901766329.17407195-199
GGGTTCG4103.5561243E-9323.60318260-264
TAGCGGT4002.9570103E-5319.73056120-124
CCCCGAA4253.884137E-6312.18192255-259
GACGGCC4250.0076213493307.99158235-239
CGTAGGT4255.490129E-5305.9383215-219
GATCGCG4300.0297.42377140-144
Produced by FastQC (version 0.11.5)