FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4118179
Sequences flagged as poor quality0
Sequence length42-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG550.00153180112308.451295-99
TATCGCG750.00259308381774.029270-274
TTACGCG400.00324572041587.0602100-104
CCGTACG850.00361029831503.5306160-164
TTCGACG1150.0061372961153.0267265-269
GATACGC1150.0064344741126.1227200-204
TTCGCGC1350.00822715995.8025280-284
GTCGGTA1400.008847636960.23816280-284
TCGGTCG1350.00886626959.2898200-204
GCGGTAT2300.009347172933.25085295
CGAGTTA4208.2218074E-5640.15875280-284
CCTTACG3454.0434497E-7622.1673295
GCCGGAC4108.9946414E-5621.37115140-144
CCGGACG2151.0426205E-4592.4702140-144
CGAGCGT1451.0811544E-4585.6602125-129
CGATAAC2400.008092476539.60046215-219
TCCGGTC5401.8575932E-4487.77448250-254
ATACCGG2750.003212647469.35953185-189
CCCGGAA9152.0737492E-4469.1753295
CGCGACC1708.300537E-5400.94153290-294
Produced by FastQC (version 0.11.5)