FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2650100
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG1404.188772E-4106.56314240-244
CGAGTAG16200.066.5015263
TACTCGG13550.064.9310153
CTACTCG13550.064.303592
AGTAGCT64600.064.298535
CCGAGTA17900.061.4333952
GTAGCTG68050.060.6869166
GAGTAGC42500.060.414684
GCTACTC31100.060.09971
TAGCTGG71000.060.019977
GCTGGGA128950.057.0897149
TTGGGAG90650.055.1306086
AAAGTGC62500.053.722594
AAGTGCT64150.052.900595
CCCGAGT21450.052.6747971
CCCAAAG76200.051.9759861
CCAAAGT71650.051.1587752
ACTCGGG17850.050.9658744
GGAGGCT94750.050.786819
GCACTTT70700.049.983911
Produced by FastQC (version 0.11.5)