FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104564
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG12450.054.763953
CTACTCG12300.053.3200042
CGAGTAG15500.052.9344943
CCGAGTA15750.051.2213632
AGTAGCT59100.050.839115
GAGTAGC37500.050.6928524
GCTGGGA115700.049.890089
TTGGGAG87150.048.8632286
GCTACTC30050.047.906491
GTAGCTG60900.047.7235536
TAGCTGG63800.046.6410837
CCCGAGT18700.045.63131
AAAGTGC63800.045.192044
GGAGGCT86850.045.0402649
CCCAAAG81200.044.536811
CCAAAGT72650.044.257922
AAGTGCT68450.043.4726225
CTCGCGT1350.003030474543.239872250-254
GCACTTT70750.043.0744741
AGTGCTG70950.041.4521876
Produced by FastQC (version 0.11.5)