FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104564
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT1059.262586E-487.28754280-284
GACGATA2550.001938736472.45868285-289
TACTCGG15550.070.904913
CTACTCG16000.068.9107062
GAGTAGC38150.058.7182964
AGTAGCT60850.058.1662675
GCTACTC35850.057.499541
GCTGGGA125700.056.910569
AAAGTGC66850.055.0141374
CGAGTAG15250.054.887253
GTAGCTG63450.054.7906156
CCGAGTA15800.054.4380382
TAGCTGG66550.053.991297
CCCAAAG83950.053.9238241
TCGCGAA1053.063833E-753.512806235-239
TTGGGAG98250.053.4872366
ACTCGGG20700.052.993134
CCAAAGT76800.052.4647372
AAGTGCT69300.052.3236125
TCGGGAG24100.051.758066
Produced by FastQC (version 0.11.5)