FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169477
Sequences flagged as poor quality0
Sequence length37-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG550.00196341432038.4672280-284
AGTCGCG950.00634969441133.4679185-189
TCCGAAC2001.6186103E-51100.5474250-254
CGACACG1050.00715380351067.7684285-289
CCGTCGG1050.00715380351067.7684285-289
CGCATCG1100.0081483741000.4976255-259
TCGATAC1150.008580813974.919280-284
CGCGTGA1100.008759464965.01904150-154
CGGTAGA2302.4885385E-5953.49225240-244
TGACGCG1150.009169958943.12823220-224
CCGTCGA600.00971452917.1228265-269
ACGTCGC1200.009696655917.1228260-264
CGTCGCG600.00971452917.1228260-264
TGCGACG1200.009839989910.42847230-234
ATGCGCG1200.009984375903.83124215-219
CTCGCGT2000.005453224528.885130-134
TCGCAAC2050.0064234952519.65784155-159
GCATCGT4351.6262624E-4509.74695275-279
TCGTTAC1302.854445E-4423.67688265-269
TAGGCGT2651.3034142E-4409.28204210-214
Produced by FastQC (version 0.11.5)