FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901044
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21250.078.641823
CCGAGTA22500.076.668732
CTACTCG19500.076.4844362
AGTAGCT78200.074.9243245
GAGTAGC50850.074.57294
TACTCGG20200.073.537463
GTAGCTG80250.072.421896
GCTACTC43800.069.764051
TAGCTGG86650.069.513327
CCCGAGT26250.068.2460561
GCTGGGA163550.067.9605949
AAAGTGC77950.066.860774
AAGTGCT79650.066.4901355
GCACTTT86450.065.147611
TTGGGAG119300.064.988576
CCAAAGT84600.064.49952
CCCAAAG94450.063.245391
CAAAGTG83950.062.929783
ACTTTGG92050.061.426613
CACTTTG91600.061.009082
Produced by FastQC (version 0.11.5)