FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062980
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA450.0065935142148.56326290-294
TGGTACG2850.002257458969.72892285-289
CGAGTAG14000.065.758953
AGTAGCT58050.060.8143775
CTACTCG14800.060.667512
GAGTAGC36650.058.776554
GCTACTC34650.058.069221
CCGAGTA15750.057.7298242
TACTCGG15950.057.3631633
GCTGGGA128000.056.2390149
GTAGCTG61650.056.168576
TTGGGAG96700.055.155086
CCCAAAG86800.054.0231821
AAAGTGC70350.053.969854
CCAAAGT78050.053.73342
CCCGAGT17600.053.609451
TAGCTGG67950.053.1548047
AAGTGCT72000.052.742525
GGAGGCT102050.051.4690069
CAAAGTG77850.051.17143
Produced by FastQC (version 0.11.5)