FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4436559
Sequences flagged as poor quality0
Sequence length45-301
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGCG409.5651636E-42922.1028285-289
CGTCGCG500.00156171572286.863255-259
GTCGCGA300.00225180151905.7194255-259
CGTACGC650.00258194381778.4565270-274
CGCGGTA700.00314443881611.5773215-219
ACGCGAA400.0044269231359.1176155-159
CACGCGA850.004990971279.1694155-159
CGCGTAG900.0051973391253.4489225-229
ACCGACG900.0056242251204.9907125-129
CGTAACG900.0056242251204.9907130-134
TCGCGTC1000.00697893371081.7039120-124
AGCGCGA1150.008258362994.2884255-259
TTCGCCG1150.008258362994.2884260-264
GTCGCGT600.008616709974.0343285-289
CGCGAGC1254.4339683E-4919.74384265-269
TGCGCGG1850.0031796978584.70483110-114
GACGGGC4652.1456389E-4465.24896100-104
ATAGCCG2700.0027086472401.6636125-129
AACTCGG5603.745226E-4386.3228120-124
TGTGCGG5405.6983867E-5340.27396295
Produced by FastQC (version 0.11.5)