FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4436559
Sequences flagged as poor quality0
Sequence length45-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG409.969407E-42862.305275-279
CCGTACG450.00130347522503.2346240-244
CCGCGAT650.00264658571756.6188265-269
TACGACG750.00387785861451.2258175-179
ATACGCG400.00451005761346.5343100-104
TTACGCG450.0057076891196.9193105-109
TCGTACG500.00596744471170.5068290-294
ACGTCGC950.0060923241157.7329200-204
CGCGTTT950.00635112261133.9236110-114
TTCGTCG1050.0070941931072.8148235-239
CACGCGA1050.0077182211028.5751145-149
TTTCGCG1150.00823676995.58435275-279
TCGCGAC600.008882665959.344280-284
TCGTCGC1200.009365058933.6931220-224
CCCGTCG1850.009616317920.7679295
GGTTCGA3200.009759015532.3189295
CGAGCTC5954.171916E-4372.6303210-214
CTAAACG3100.007776048362.39856230-234
CCGTAGG3200.009833512341.9072190-194
CGACATC3306.1796163E-4327.27386130-134
Produced by FastQC (version 0.11.5)