FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3257520
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTC1806.993601E-4314.15405200-204
ATATCGC2350.0011474326266.25772275-279
ATCGTAC2250.00146126245.63124110-114
GGTCGGT2950.0027836203198.04149235-239
GTCGGTA1750.004642304166.99756235-239
GGTTCGT2153.9673917E-4148.74004285-289
TGAACGC4350.008341476137.2099250-254
ACTCGCG2100.0061911256134.39787195-199
TATCGAC2251.015851E-6134.27841260-264
CTCGCTA2100.009429757131.76033125-129
CCGTAAG2408.8218047E-4118.22926205-209
ACGGACC2455.936899E-5116.23228210-214
CCGTTGG4300.006427445112.2908295
AATTGCG1553.6945424E-4109.97651205-209
CGTAAGG2805.249058E-799.12285160-164
CTAATCG1455.043777E-495.28798100-104
TAGGCGT3203.7289283E-1092.39071245-249
TAAACGG3500.001213084191.36887285-289
GATTACG3553.6445785E-585.10603260-264
CGCAACG1150.00817750884.74795235-239
Produced by FastQC (version 0.11.5)