FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3257520
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTA2450.0016175605237.422235-239
ATCGCCG1300.0021221023216.939200-204
GTAACCG1450.0021310546216.62312285-289
TGGTCGG4600.0028267463196.92612295
GCCGACC3800.004957082163.2897280-284
GCGCGAC1500.0076985927141.02608290-294
CGCAGAT4400.007889198139.78828275-279
CCGACGT1600.009336629132.21194290-294
CCCGACG2401.7736533E-4132.21194290-294
CCGTTAT2250.00997028129.32257235-239
ATAGCGG3757.526887E-7120.78136295
CGGGTTA2500.009523608120.55108265-269
CCCGAAC2306.757273E-4120.04754145-149
ACGGATA2700.0040741046106.83468225-229
TAAACCG2650.00322223104.19221140-144
GCGGTTA3051.3734498E-6101.72145280-284
CTAAACG3100.006365944101.67729290-294
CCGATCA2850.0033982815101.4896230-234
CTCTACG3254.434974E-594.625916275-279
GACCGGT3353.6437268E-691.80126275-279
Produced by FastQC (version 0.11.5)