FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3328887
Sequences flagged as poor quality0
Sequence length38-301
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT3052.2068596E-4461.0762295
CTTATCG2858.050054E-4299.60745250-254
TATTCGG2858.3758106E-4295.67044240-244
ATAGCCG1900.003426561213.0731140-144
CTCGCGT2103.6066928E-4208.01375280-284
CGTCCCT4200.002923799194.74757190-194
ATTCGGT3253.7946687E-5194.58801240-244
AACGATC2553.818426E-4162.45245215-219
AATGGCG5900.0073909615142.82385240-244
ATGCGCC3100.009388834131.92578185-189
TACTCCG1952.059029E-4127.36395210-214
GGGTACG3204.6959867E-5126.6721165-169
TAGCGGT3356.3698826E-5122.08056185-189
CGGCCTA3502.1142114E-8118.97305225-229
GTACCGG1950.0040728953113.55447290-294
GCCCGGT3852.3310393E-4105.68672175-179
GCCGGTT4258.403731E-10103.63038285-289
GACGGGT4253.3829383E-6103.63038285-289
GAATAGT12804.8767118E-4102.38177280-284
GGCGGTT4400.008827408100.65055290-294
Produced by FastQC (version 0.11.5)