FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3328887
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT454.750519E-4357.6761100-104
CGTGAAT4750.001056418273.4225295
GCCGCGT3200.001146405266.24512265-269
GTCGTAT1655.7735422E-5252.45479235-239
CACGGCG3800.001812395228.4712290-294
ATACGCG800.0026604712201.19278100-104
TATTGCG2200.0023361112182.90254130-134
CGGTGCG2405.5868753E-5177.49675265-269
GTTACGC1200.0045379032168.29654155-159
GGCTACG2605.073953E-4162.97397255-259
GCTACGG2750.0028208059154.90627265-269
TGCTACG2650.0021399194152.61244170-174
ACGCGGG2708.524328E-6152.0966205-209
CCGCGGA2950.004757763146.75217285-289
CCCGGGT9300.0139.65126295
TGACGCG1200.008508414136.39607200-204
GGGTGCG6500.009438593131.60094270-274
ACTCCGG5203.2741813E-5124.88046295
CCCGTAG3608.669802E-6117.39203250-254
GCGAGTG3601.2815988E-4112.19769145-149
Produced by FastQC (version 0.11.5)