FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3131829
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG550.003967518176.05836165-169
CGGCGTA850.007677728141.17812280-284
CGAGTAG15350.053.727893
AGTGCGA3400.00639583553.60905285-289
CTACTCG11250.053.2435042
TACTCGG11350.053.0799453
AGTAGCT59400.052.97675
CCGAGTA16750.052.9396552
GAGTAGC35650.052.338374
GTAGCTG61250.051.4720846
GCTGGGA119050.050.556279
GCTACTC26150.049.41441
CGACTCG2500.001057553349.391876290-294
TAGCTGG66750.049.0711067
TTGGGAG80300.048.511846
CCCGAGT19650.047.8255461
AAAGTGC63400.044.1911544
CCCAAAG79950.044.0058981
AAGTGCT67100.042.8004235
GGAGGCT83650.042.653339
Produced by FastQC (version 0.11.5)