FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3131829
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG14750.066.222853
CCGAGTA15950.060.8728752
GAGTAGC34850.057.2360534
TACTCGG13550.056.979423
AGTAGCT56350.055.8496325
CTACTCG13950.055.344722
GCTACTC32350.054.25261
GCTGGGA125500.054.19929
AAAGTGC69100.054.0083244
GTAGCTG58600.053.7138186
CCCGAGT19200.052.713091
CCAAAGT74450.052.6364442
TTGGGAG98150.051.9193466
CCCAAAG83150.051.6425171
TAGCTGG64350.051.018397
AAGTGCT72850.050.8283655
CAAAGTG74150.050.56273
GGAGGCT97350.050.2690669
CGATAGC1608.16799E-847.31725240-244
CCGTACG505.797676E-446.801911
Produced by FastQC (version 0.11.5)