FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728436
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG15350.078.029742
AGTAGCT88550.077.227795
CGAGTAG25100.075.652463
GTAGCTG90350.075.192216
TACTCGG16850.073.859583
ACTATCG2156.0743077E-573.85748250-254
GAGTAGC58600.073.456444
CCGAGTA27000.072.743082
TAGCTGG98200.071.5331957
GCTGGGA183400.070.4474269
AAAGTGC86000.070.044194
GCTACTC38800.069.391141
AAGTGCT87500.069.13675
TTGGGAG120350.069.135156
CCAAAGT93050.067.4059452
CAAAGTG92150.066.135293
GCACTTT95250.065.9324341
CCCAAAG103850.065.620371
ACTTTGG98600.065.322353
CCCGAGT30200.064.410921
Produced by FastQC (version 0.11.5)