FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728436
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21650.084.500463
AGTAGCT86250.082.4039845
GAGTAGC52900.081.914354
GTAGCTG87950.081.109146
CCGAGTA22900.079.885052
TACTCGG22750.078.157463
CTACTCG21600.078.155252
TAGCTGG95350.077.330037
AAAGTGC88250.076.017494
GCTGGGA185400.075.681469
GCTACTC47700.075.65011
AAGTGCT90550.074.73295
CCAAAGT95850.074.3339162
GCACTTT96850.073.920831
TTGGGAG130200.072.3946846
ACTTTGG100250.071.394143
CAAAGTG97150.071.0293353
CACTTTG101450.069.787822
CCCAAAG109450.069.693491
AGTGCTG97400.068.295556
Produced by FastQC (version 0.11.5)