FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3499673
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG1850.001061932884.32991265-269
CGAGTAG21600.068.386253
CCGAGTA22500.066.14342
AGTAGCT80450.063.854685
CTACTCG15600.063.086522
GTAGCTG82200.061.7668236
GAGTAGC52700.061.6190224
TACTCGG15700.060.94613
TAGCTGG87950.059.4965257
GCTGGGA168550.058.8977559
AAAGTGC85300.057.629974
CCCGAGT27550.057.0808531
GCTACTC38900.056.7817761
AAGTGCT88300.055.8751685
TTGGGAG109500.055.4333726
TCCGAAA7351.1393255E-555.365623295
CCAAAGT99150.054.1686062
CCCAAAG110100.054.0252881
CAACGCG1450.00627936553.866936265-269
CGGGTAA4650.00694172852.50804295
Produced by FastQC (version 0.11.5)