FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2875086
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGA2056.1129103E-4328.48785215-219
CATGCCG2950.001720915232.48807240-244
CACGGCG3300.0020689925218.59865285-289
CGACGAC1700.0027855146198.05885215-219
ATAGGCG1750.007883526191.7046190-194
GCCCGGT3500.0031211798190.55669160-164
AGCGGGT3607.965196E-6185.70825175-179
AGCGGTA3950.00444989169.25308170-174
CGCTTAT2050.004832921164.74194220-224
CCGGACG1550.006390163150.08002260-264
GCGTTTA2401.5521974E-4145.02654260-264
GGCACGT4700.0074034473142.75874205-209
ACGTGCC4950.007887938139.7583255-259
GCGGTAA2400.007984206139.28119170-174
CCGGCGA1700.008182896138.16505265-269
TATCACG2550.00862407135.73207255-259
CGGTAAC1650.0012456325135.06055170-174
GCCTCGA5350.008950067133.96887275-279
TAATACG2750.009188174132.88043290-294
CCAGACG5050.00917648132.86458185-189
Produced by FastQC (version 0.11.5)