FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2875086
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG201.5793159E-51113.2537180-184
CGCGACA852.8438907E-4424.3155290-294
TTGACGG3255.756886E-4334.8828295
ATCCGGT2358.862528E-4290.1711230-234
CCGCGCG1350.008542566256.016255-259
GGCGCGC3100.0018010396228.9697275-279
AATCCGA3600.0027005626199.98138290-294
TACGAAC1900.0027091997187.51039285-289
CCGTTAG1801.086014E-4185.98991210-214
CTTAGCG1850.0028101834180.31065175-179
CGTTAGT2000.004727311178.13487285-289
TAGCGTA1000.008505346177.4515275-279
CGCCGGC4100.00398518175.5934290-294
AATCGGG3100.006615919175.54343295
TAGTCCG2002.4266263E-4166.18811160-164
TCGGTAA2000.00477615165.3958110-114
ATCGCCG1100.0053449874159.333265-269
TAACGGG2255.947719E-4151.90704235-239
CAATCGA2350.006183965151.70152285-289
TAGCCGG4800.0070784865144.91132260-264
Produced by FastQC (version 0.11.5)