FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750297
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG550.0060308604112.891495290-294
AACGCAT3507.400286E-570.960365290-294
CGAGTAG13950.059.175663
CCGAGTA15200.054.90232
GAGTAGC33000.052.1442874
CTACTCG9700.049.9349332
AGTAGCT51900.049.732995
TACTCGG10800.048.444863
GTAGCTG52850.048.179036
GCTGGGA105350.047.6769189
CCCGAGT18950.047.4321751
ACGCATT3353.2334454E-546.42621290-294
TAGCTGG56850.045.913977
TTGGGAG73750.043.6693466
GCTACTC25650.043.461781
CCCAAAG67300.042.668911
CCAAAGT61150.042.6588552
AAAGTGC54500.042.5603644
GGAGGCT71000.042.16759
CAAAGTG60450.041.1601033
Produced by FastQC (version 0.11.5)