FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750297
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGC2450.001856878773.24924290-294
CTACTCG13450.060.0073432
TACTCGG13350.059.5891233
CCGAGTA13600.057.2107732
CGAGTAG13000.057.1734433
GCGGTTA3000.001295784157.08627295
GCTACTC31800.054.6033361
GCTGGGA107000.052.0446249
AAAGTGC60250.051.8580444
GAGTAGC30550.050.756424
TTGGGAG80600.050.742386
CCCAAAG71800.050.3893471
CCAAAGT63400.050.279972
AAGTGCT63750.049.8362665
AGTAGCT51050.049.4916425
GTAGCTG50200.049.4148376
TAGCTGG53200.048.719937
AGTGCTG65250.047.899946
GGAGGCT88700.047.2504359
GCACTTT63850.047.2043041
Produced by FastQC (version 0.11.5)