FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566088
Sequences flagged as poor quality0
Sequence length36-301
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG802.7206243E-6138.03691140-144
GCTCGAA4350.001687231775.0185295
CGCCGCT4008.366859E-569.21687265-269
CGAGTAG11600.056.7802433
GAGTAGC29150.051.3703354
AGTAGCT45900.050.7758265
CCGAGTA13600.050.7232362
GTAGCTG47400.049.1689956
CTACTCG8950.048.6467362
TAGCTGG52450.047.869487
GCTGGGA94300.047.3402829
CCCGAGT15800.046.529871
AAAGTGC49100.045.8614584
TTGGGAG65750.045.806536
CCCAAAG62600.044.8980671
AAGTGCT50550.044.657315
GCTACTC21750.044.4613271
CCAAAGT56850.043.8625832
TACTCGG10250.043.3882943
ATGCCGC3008.560427E-443.234005235-239
Produced by FastQC (version 0.11.5)