FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566088
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT1450.0076100337141.56998295
TCGCGAA900.002817317177.358444280-284
CTACTCG11950.062.1420562
CGAGTAG11600.061.593353
TACTCGG12900.057.130723
CCGAGTA13050.056.9040342
GCTACTC28000.056.6724931
TTGGGAG75350.055.495096
CCCGAGT14450.055.297021
AAAGTGC55950.054.2067034
CCCAAAG67650.053.983141
GCTGGGA100250.053.9292079
GAGTAGC29000.053.551964
TAGCGGC2653.0910713E-453.154636285-289
AGTAGCT46450.052.3334355
CCAAAGT61250.052.0782742
AAGTGCT58000.051.8077245
GTAGCTG48100.050.7801136
GGAGGCT82000.049.3114439
CAAAGTG63400.048.715953
Produced by FastQC (version 0.11.5)