FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2845717
Sequences flagged as poor quality0
Sequence length38-301
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA1201.4444994E-4531.66437130-134
AGCGCGC1402.1755094E-4463.75424210-214
CCCGTAC1000.0014279411322.72958195-199
ATTACGG2850.0016909455233.86203250-254
GCGCTAC1450.0017845269229.82993250-254
ATCGGTC1750.0077877976192.99443265-269
AGCGGTC3400.003268682187.64624125-129
AGCCCGC3950.004671752166.52362230-234
CAAACGA4250.0063494267150.29074100-104
TAGCGAA1550.0068152393146.88268275-279
CACGCAA2200.008666173145.16719140-144
CAATGCG2558.324807E-5127.75599220-224
ATCGGGT2750.0026328878122.81465265-269
CGTAGCT2950.00445177114.48823265-269
TATCCGG2103.2083597E-4113.92808115-119
CCGTAAA3150.0070013064112.017944290-294
TACGGAG3100.0064416123110.710075280-284
GCATAGA9956.1884546E-4108.540276295
ATAGGCG3251.4191519E-6105.60038280-284
AACGCAC3401.09980036E-4103.781334290-294
Produced by FastQC (version 0.11.5)