FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2845717
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT350.009439217930.7101225-229
CGTACGG350.009551413925.2288220-224
GGTACGC955.0483586E-4350.36545260-264
ATGCACG2700.0013213773253.93161290-294
TGCGGAC1750.0032713895187.69876235-239
GTCCGGT2902.6334633E-4181.2697295
CTACCGT1800.00391391176.78224135-139
GCCGTCA3900.0039718603175.7988290-294
GAAATCG4000.0042843563171.40384290-294
TATGGCG3250.009346442161.74835295
GCATCGC2100.0058688107154.38666220-224
CGCTACG1150.0063089924150.73839290-294
TCGCGTC900.0076096705141.58968145-149
TCTACGC1250.005450739136.10483285-289
CCGCGCG2550.0016939713133.10664285-289
AATCGCC2450.0012793986130.9417190-194
CGAACAT2700.004324118124.78746280-284
TTGGGCG4250.0064210193123.68992295
CCCGTAG3051.4514098E-6110.197495275-279
TATTCGG3054.0579255E-4109.13021260-264
Produced by FastQC (version 0.11.5)