FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350921
Sequences flagged as poor quality0
Sequence length42-301
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAA2255.5833446E-4338.5871205-209
ACCGTAT3956.007875E-4330.1276295
GCGATTG2608.1026804E-4298.99503245-249
ACGGTCG1350.008587913279.522175-179
GCGTAGA1650.0012796764228.69983180-184
GACGTAC1750.00817419227.58128265-269
ACCTCGC3900.002256828212.34644285-289
AATGCGC2200.004261373171.82562185-189
CCGGGTG7853.8873623E-8166.11516295
GGTTACG2350.005050847163.82623230-234
CGGTAGT2601.0648432E-4155.86708275-279
GCGATAG2450.008226925153.66373160-164
CCGGTTA2450.0011437241152.59785105-109
TACGAAC2550.006477252149.37666205-209
TGACGGA5400.0074300654142.58951230-234
CGAACTA1400.008574278136.03946205-209
GGGTACG3005.86129E-4130.34785250-254
GGCCGTT3003.5842526E-4126.374756195-199
GGTTCGT3102.744901E-5122.441696200-204
GGCGGTT3305.4308806E-5121.4348270-274
Produced by FastQC (version 0.11.5)