FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350921
Sequences flagged as poor quality0
Sequence length42-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCG1051.7252241E-6358.32224175-179
ATCGCCG1100.0026724706342.03488170-174
ACCGTAT3706.0329435E-4329.70932295
CGACCGT1350.008702871294.1591270-274
CGGTAGT1750.0020012886221.1951245-249
GTACGAT1850.0020391934219.80621285-289
GCGCACT3750.0024647573206.2009245-249
TAACCGC1950.002929091194.76297210-214
TAATCGG2101.7568538E-5193.6388285-289
GTACGGG2100.0032465677188.17415265-269
ACGCAAA4150.0035337799182.81468205-209
CGTAGGG2358.5501536E-4172.82071285-289
TATCGTC2500.0049166256163.79405290-294
TCAATCG2350.005007808162.76346225-229
AACGTAG2500.0053893295158.8459270-274
CTACGAG2550.0015843314150.35483230-234
CCGCCTT8250.0066507584147.86963295
GGTTCGT2751.7444392E-4140.59154245-249
GACCGAA2801.1768154E-5140.27005260-264
TCGTACA2901.4574631E-4138.87685280-284
Produced by FastQC (version 0.11.5)