FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712191
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG15500.068.894943
CCGAGTA16250.068.6297452
CGATATG3151.1735452E-464.65533290-294
CCCGAGT19400.060.3885081
GAGTAGC35950.059.580414
AGTAGCT60750.059.5421185
GTAGCTG60850.059.2413866
CTACTCG10850.057.6746442
TAGCTGG64700.057.338087
GCTGGGA116500.056.9051139
AAAGCGC9201.8189894E-1255.34355290-294
TACTCGG12250.053.9163673
TTGGGAG86050.052.724136
AAAGTGC61400.051.271354
GCTACTC29100.050.696521
AAGTGCT64150.050.420555
CCCAAAG75350.048.0423051
ATTCGCG850.006027521647.862774180-184
GGAGGCT83000.047.6708539
CCAAAGT70100.047.1972272
Produced by FastQC (version 0.11.5)