FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089224
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAC2550.001831859973.50136280-284
CTACTCG15250.061.8216132
TACTCGG15650.060.244443
CGAGTAG14600.059.0644043
CCGAGTA15250.055.7902372
GCTACTC32300.054.652211
GCTGGGA114700.053.7314839
AGTAGCT55850.053.3334355
TTGGGAG89000.053.2450756
AACTACG3600.006735530752.911156290-294
GTAGCTG56800.052.6498646
GAGTAGC36650.051.301684
TAGCTGG60050.051.0553137
AAAGTGC63250.050.7260974
AAGTGCT65950.049.438045
GGAGGCT93400.049.2269179
CCCAAAG80750.048.7063331
CCCGAGT18300.048.3884281
CCAAAGT74500.047.6868362
TCGGGAG20650.047.3446736
Produced by FastQC (version 0.11.5)