FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3254641
Sequences flagged as poor quality0
Sequence length48-301
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG800.00203163432002.5004295
TCGCGTA550.00253054641796.027285-289
CGCGTCG650.00407340561415.687135-139
CGTCGAA700.00441939571359.0596235-239
CACGCGT700.00444666951354.8906220-224
ACGCGAA700.0047522121310.6656100-104
CGCGTAT450.006763591099.3282285-289
CGTACCG450.00707014461075.2427260-264
ACGCGTA450.00736088261053.8038220-224
CGACCCG900.0075770451037.8854175-179
TAGGTCG1252.2571831E-4782.7495270-274
GCGACGT1052.6049046E-4436.88858110-114
CTCGAAA4703.3721444E-4399.90433200-204
CGACGTC1055.085606E-4349.5109110-114
GCGTGTA2750.0029670673336.62064145-149
CGCGGGC2950.0051865564325.49326240-244
CGTGTAG2902.9622368E-4317.30914120-124
TGTACCG3100.007500628309.74356240-244
GTACGGC3002.57004E-5305.822110-114
TGCCGGT3101.1183329E-7300.41397165-169
Produced by FastQC (version 0.11.5)